Reliable fluorescence measurements are essential for quantitative analyzes in bio- and life-science. However, quantitative fluorescence measurements can be distorted by wavelength and time-dependent instrument contributions, resulting in measurements that are not comparable across instruments, laboratories, and over time. In order to obtain comparable and instrument-independent fluorescence data, it is important to determine and adjust for the bias of the instruments and rule out that this is the dominant source of variation.
DFM will address this challenge by establishing metrological traceability chains for fluorescence measurements using. physical and chemical reference objects and calibration methods. Based on DFM’s ISO17025 accredited laboratory in the field of optical radiometry, stable reference standards and methods are developed to perform valid, quantitative fluorescence measurements that can be traced to the laboratory’s primary standards in spectral radiance and responsiveness.
Our goal is to help ensure the accuracy and reproducibility of fluorescence measurements and ultimately standardization in highly regulated areas. This activity is funded with support from the Danish Agency for Education and Research. If you want to hear more, you can contact researcher Olga Rodenko (oro@dfm.dk).